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Full-time
bachelor degree
Posted 8h ago
~40 hrs/week
Responsibilities
The Research Technician will perform extracellular vesicle purification, genetic engineering of cell lines, and sample processing for downstream analyses like proteomics and RNA sequencing. They will also assist with laboratory organization, equipment maintenance, and reagent stock management.
Requirements
A Bachelor’s degree in biology, chemistry, or a related field is required along with proficiency in adherent mammalian cell culture. At least one year of related laboratory experience and experience with biochemical purification are preferred.
Full job description
We are particularly looking for a motivated, energetic, flexible, organized, and independent individual.Key activities will include CRISPR-based genetic engineering and culturing of adherent mammalian cell lines, biochemical purification and characterization of EVs, mouse tissue harvesting and processing, Western blotting and other experimental techniques in support of ongoing research projects. The successful applicant will have demonstrated proficiency with adherent cell culture. Experience with biochemical isolation and purification is preferred.The Research Technician will also work alongside other research staff in the laboratory to facilitate ordering, equipment maintenance and training, and laboratory organization. This is a full-time, hourly position. Very limited weekend hours may be required on occasion. The applicant will report directly to Dr. Alissa Weaver and will work closely with senior members of the laboratory.
The Research Technician will be part of the Weaver laboratory in the Cell and Developmental Biology Department at Vanderbilt University.This Research Technician is responsible for extracellular vesicle (EV) purifications and quality control analysis, development of new cell lines for cancer-related EV studies, and sample processing for downstream analyses (imaging, proteomics, lipidomics, RNA sequencing).Much of the work relates to a project on how RNA is trafficked into EVs and how those RNA-containing EVs drive colon cancer aggressiveness.
Key Functions and Expected Performance:
Extracellular Vesicle purification and analysis
Adherent Cell Culture
Genetic engineering of cell lines
Carrying out experiments under supervision
Proactively troubleshooting and seeking out advice/feedback
Maintaining accurate experimental records
Meeting at least once a week with Dr. Weaver and/or laboratory subgroups to present data and report on the week’s activities
Maintaining equipment and reagent stocks
Organizing laboratory protocols in shared online databases
Qualifications:
A Bachelor’s degree in biology, chemistry, or a related field of study from an accredited institution of higher education is required.
Experience performing adherent mammalian cell culture is required.
Experience performing biochemical purification is preferred.
At least one consecutive year of related lab experience is preferred, either in undergraduate research or in a post-graduate technical position.
Strong organizational, oral, and written communication skills are required.
Supervisory Relationships:
This position does not have supervisory responsibility; this position reports administratively and functionally to the Principal Investigator of the laboratory, Dr. Alissa Weaver.
At Vanderbilt University, our work - regardless of title or role - is in service to an important and noble mission in which every member of our community serves in advancing knowledge and transforming lives on a daily basis. Located in Nashville, Tennessee, on a 330+ acre campus and arboretum dating back to 1873, Vanderbilt is proud to have been named as one of “America’s Best Large Employers” as well as a top employer in Tennessee and the Nashville metropolitan area by Forbes for several years running. We welcome those who are interested in learning and growing professionally with an employer that strives to create, foster and sustain opportunities as an employer of choice.
We understand you have a choice when choosing where to work and pursue a career. We understand you are unique and have a story. We want to hear it. We encourage you to apply today so that you might become a part of our story.
Vanderbilt University is an equal-opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran, or any other characteristic protected by law.
A unique multidisciplinary experience encouraging collaboration between engineering, chemistry, physics, and medicine.
Industry
Higher Education
Company size
5,001-10,000 employees
Headquarters
Nashville , TN
LinkedIn followers
1,147
Our Interdisciplinary Materials Science (IMS) program offers a unique multidisciplinary experience where students design their own curriculum, and collaborate with faculty and students from engineering, chemistry, physics, and medicine, who are working on some the biggest of society's challenges.
The IMS Ph.D. program is integrated with VINSE (Vanderbilt Institute of Nanoscale Science and Engineering), which boasts brand new state-of-the-art fabrication and characterization facilities. Resources include a cleanroom, analytical/nanocrystal support core, and an advanced imaging suite.
Our students rotate through three different labs in the first year to learn the culture of each research group and the advising style of the mentors prior to project/adviser selection. We offer a highly customized curriculum where students can select courses from a variety of technical departments to support their research project. This is work is supported by research facilities equipped with the latest instrumentation with outstanding research programs and faculty, where IMS researchers use nanotechnology to address the world’s most important challenges in energy, health, and security.
Our graduates work in the government (FDA, NIH, US patent office), in industry (Google, Johnson & Johnson, Dow, DuPont, Exxon, and Biotech start-ups), and in academia.
For more information contact:
Josh Caldwell, IMS Director
[email protected]
Sarah Ross, Program Manager
[email protected]
A unique multidisciplinary experience encouraging collaboration between engineering, chemistry, physics, and medicine.
Industry
Higher Education
Company size
5,001-10,000 employees
Headquarters
Nashville , TN
LinkedIn followers
1,147
Our Interdisciplinary Materials Science (IMS) program offers a unique multidisciplinary experience where students design their own curriculum, and collaborate with faculty and students from engineering, chemistry, physics, and medicine, who are working on some the biggest of society's challenges.
The IMS Ph.D. program is integrated with VINSE (Vanderbilt Institute of Nanoscale Science and Engineering), which boasts brand new state-of-the-art fabrication and characterization facilities. Resources include a cleanroom, analytical/nanocrystal support core, and an advanced imaging suite.
Our students rotate through three different labs in the first year to learn the culture of each research group and the advising style of the mentors prior to project/adviser selection. We offer a highly customized curriculum where students can select courses from a variety of technical departments to support their research project. This is work is supported by research facilities equipped with the latest instrumentation with outstanding research programs and faculty, where IMS researchers use nanotechnology to address the world’s most important challenges in energy, health, and security.
Our graduates work in the government (FDA, NIH, US patent office), in industry (Google, Johnson & Johnson, Dow, DuPont, Exxon, and Biotech start-ups), and in academia.
For more information contact:
Josh Caldwell, IMS Director
[email protected]
Sarah Ross, Program Manager
[email protected]