Site: The General Hospital Corporation

 

Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.

 

 

Job Summary

A Research Technician II position is available in the lab of Dr. Christopher Mount in the MGH Pathology Department and Krantz Family Center for Cancer Research. This position requires an individual who will work independently under supervision to generate and evaluate cellular immunotherapies in brain tumor model systems. With training and supervision, this individual will also perform molecular characterization assays including single cell sequencing, spatial transcriptomics, and multiplexed immunofluorescence. This position will also require entry-level laboratory management, including monitoring of inventory, package receiving and shipping, routine instrument maintenance, and interfacing with core facilities and collaborators to facilitate specimen handling.

The lab develops and evaluates cutting-edge cell immunotherapies in model systems and patient samples at the interface of fundamental biology and cellular engineering with the ultimate goal of developing translatable insights to improve outcomes for brain tumor patients. In addition to the opportunity to contribute to this mission, this role offers the chance to present at laboratory meetings and departmental seminars, and dedicated prior technicians have presented their work at national scientific conferences and co-authored publications. Enthusiasm, work ethic and eagerness to contribute as part of a larger team are valued over specific skills; however, a documented record of at least two years (post-undergraduate) in an academic or industry laboratory setting is required. The role is a great opportunity for those looking to develop as a scientist and prepare for graduate or medical school. Alternatively, those interested in a longer-term commitment will find opportunities to advance in scientific or management roles within our team.

 

• Generate and maintain CAR-T cells, including production of viral stocks, collection of primary T cells, cell transduction and expansion, and maintenance of associated records
• Cell culture of diverse human cell lines, including brain tumor models and patient-derived organoids
• Assay the activity of CAR-T cells in vitro, including cytokine generation, cytotoxicity, and cellular imaging
• Flow cytometric analysis of CAR-T cells
• Molecular cloning, including coordination and assessment of sequencing results and maintenance of documented records of constructs
• Execution of protocol-based genomics assays including single cell sequencing, spatial transcriptomics, and spatial proteomics
• Interface with collaborators and service core providers to transfer specimens, submit service requests, and oversee receipt of data and services. This may involve travel to local MGB hospitals to collect patient tissue specimens (shuttles available at no cost).
• Basic laboratory management including inventory management, package receipt/shipping, routine instrument monitoring and maintenance, maintaining and distributing key lab protocols.
• This role requires being listed as an emergency contact alongside other team members for mission-critical instrumentation (such as scientific freezers). In the unexpected event that such instrumentation fails, this may result in after-hours contact and a responsibility to help coordinate a response alongside other team members.
• Attendance and presentation at weekly lab scientific meetings.

Education
Bachelor's Degree Related Field of Study required

Can this role accept experience in lieu of a degree?
No

Experience
2+ years of post-undergraduate experience in an academic or industry biosciences laboratory setting are required (with 2 references)

Knowledge, Skills and Abilities

• An ability to clearly articulate one’s role(s) in this past experience, including scientific question or goal, and how one’s work contributed to the efforts of the larger team
• Enthusiasm, eagerness to learn and develop as part of a collaborative team
• Ability to work independently and rigorously document one’s work
• Excellent written and verbal communication skills
• Strong organizational skills and attention to detail

• The following specific experience is additionally considered valuable but is not required. Any candidate with experience in 1+ of these areas is encouraged to apply:
• Experience with CAR-T cell production and relevant in vitro assays
• Experience with molecular cloning, including validation of generated constructs
• Prior mammalian cell culture work, including lentiviral production
• Previous work performing single cell sequencing, spatial transcriptomics, or immunofluorescence microscopy
• Experience with histology, including specimen preparation, cryosectioning or FFPE sectioning, and immunostaining
• Software: Microsoft suite including Excel, Graphpad Prism, Flowjo, Snapgene or other molecular cloning software

 

 

Onsite

 

149 Thirteenth Street Building 149

 

40

 

Regular

 

Day (United States of America)

 

Pay Range

$24.28 - $39.43/Hourly

 

Grade

6

 

At Mass General Brigham, we believe in recognizing and rewarding the unique value each team member brings to our organization. Our approach to determining base pay is comprehensive, and any offer extended will take into account your skills, relevant experience if applicable, education, certifications and other essential factors. The base pay information provided offers an estimate based on the minimum job qualifications; however, it does not encompass all elements contributing to your total compensation package. In addition to competitive base pay, we offer comprehensive benefits, career advancement opportunities, differentials, premiums and bonuses as applicable and recognition programs designed to celebrate your contributions and support your professional growth. We invite you to apply, and our Talent Acquisition team will provide an overview of your potential compensation and benefits package.

 

1200 The General Hospital Corporation is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran’s Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at (857)-282-7642.

 

Mass General Brigham Competency Framework